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. 2020 Apr 22;8:262. doi: 10.3389/fcell.2020.00262

FIGURE 2.

FIGURE 2

Suppressive effect of HJ901 on TLR7/9-mediated cytokine production in human PBMCs. (A,B) Human PBMCs were cultured with 10 μM IMQ or 1 μM CpG in the presence or absence of different concentrations of HJ901 (0.1, 0.5, 2.5, or 12.5 μM or 0.1, 0.3, 0.9, or 2.7 μM) for 36 h. (C,D) Human PBMCs were cultured with HJ901 in the presence or absence of different concentrations of IMQ (10, 30, 90, or 270 μM) or CpG (1, 3, 9, or 27 μM) for 36 h. (E,F) Human PBMCs were incubated with 10 μM IMQ or 1 μM CpG for 0, 0.5, 1, 2, 12, or 24 h and then treated with or without HJ901 for 36 h. Supernatants were analyzed for IL-6 and IL-10 levels with a cytometric bead array (CBA). Similar results were obtained from three independent experiments. All data are presented as the means ± SEM (n = 5 in each group). ##p < 0.01 vs. the untreated group or HJ901 group; *p < 0.05 and **p < 0.01 vs. the IMQ or CpG group.