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. 2020 Apr 28;10:7100. doi: 10.1038/s41598-020-63517-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative images of the wound healing assay and the high content screening of closure responses using Oris Pro assay. Representative CM-Dil-20 stained images of cMSCs are shown at t = 0 for non-treated (A) and ClGBI-treated (100 µM) wells (B). Images were taken immediately following cell seeding. The final images of the closure areas (t = 41.3 h) are in panel C (control) and D (treated) respectively. Intensities were rescaled, scale bar represent 1 mm. Diagrams present open area (cell free zone) of ClGBI-treated (100 µM, F) and non-treated (control; E) cMSCs at t = 0 and t = 41.3 hours. Each bar represents the average and SEM of 7 wells (n = 7). G, Comparison of closure responses of MSCs following treatment with ClGBI (100 µM) over 41.3 hours versus control non-treated MSCs. Closure% = [(initial cell free are − cell free are at a given time pont)/initial cell free area] * 100). ***p < 0.0001 where indicated versus non-treated control. H, Time-dependence of the closure responses of MSCs in the absence (Control, black) and presence of 100 µM ClGBI (Treated, red). Each time point represents the average percent closure and SEM of samples from 3 different placenta donors.