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. 2020 Mar 17;122(9):1391–1398. doi: 10.1038/s41416-020-0794-x

Fig. 2. Effects of serine availability on tumour cell growth.

Fig. 2

a G55 cells were incubated in serum-free medium without glucose restriction (25 mM) depleted for glycine and serine with vehicle or 60 µM CBR-5884 as indicated for 24 h. Serine, glycine, asparagine and tyrosine levels were measured by LC-MS/MS (n = 3, mean ± SD, n.s. not significant, *p < 0.05). b LN-308, LNT-229 and G55 cells were incubated in DMEM (25 mM glucose) depleted for glycine and serine with or without CBR-5884 as indicated. Serine was replenished as indicated, with (upper panel) and without (lower panel) 10% FCS. Cells were incubated for 4 days. Cell density was measured by crystal violet staining at the beginning of cultivation and at 4 days (n = 6, mean ± SD, n.s. not significant, *p < 0.05, **p < 0.01). C, LN-308, LNT-229 and G55 cells were incubated in serum-free DMEM depleted for glycine and serine with or without CBR-5884 as indicated. Cell death was quantified by PI-uptake after 4 days (n = 3, mean ± SD).