Figure 2.

The Effect of X19-mu Treatment on Lesion Histology in Aortic Root Sections

(A) Atherosclerotic lesions in the aortic root stained with vascular cell adhesion molecule (VCAM)-1 (green), (B) intracellular cell adhesion molecule (ICAM)-1 (green), (C) interleukin (IL)-1β (red), (D) monocyte chemoattractant protein (MCP)-1 (red), as well as (E) Mac-3 and cleaved caspase-3 (yellow). All sections are counterstained with 4',6-diamino-2-phenylindole (blue, nuclei). (F) Lesion collagen content is quantified from Masson’s trichrome stainings (blue). Quantitative results are shown as individual data points in addition to mean ± SD in histograms; Fisher’s exact test for histological scores (A and B), and Student’s t-test for unpaired measurements (C to F); n = 10 to 16/staining/group. Scale bar = 100 μm (10 μm in inserts in E).