Figure 6.

X19-mu Treatment Reduces ¹⁸F-FDG Uptake in Atherosclerotic Lesions

(A) A hematoxylin and eosin−stained longitudinal aortic cryosection, (B) corresponding autoradiograph, and (C) macrophage (Mac-3) staining. Black lines represent contours of the regions of interest defined in the atherosclerotic lesions and vessel wall without lesions. (D) The graph shows average 18-F-fluorodeoxyglucose (¹⁸F-FDG) uptake in atherosclerotic lesions (normalized by the activity of vessel wall; lesion-to-wall ratio), which was lower after a 6-week treatment with X19-mu than with vehicle. (n = 16 mice/group; mean ± SD; sex-adjusted model). (E) The subanalysis showed that X19-mu treatment reduced ¹⁸F-FDG uptake (lesion-to-wall ratios) in lesions with low, intermediate, and high macrophage density compared with vehicle. (n = 5 to 7 mice/group in each subcategory; mean ± SD; 2-way analysis for variance for the main effects of macrophage density and treatment). Scale bar = 0.5 mm. A = arch; AA = ascending aorta; B = brachiocephalic artery; D = descending thoracic aorta; L = lesion; LC = left common carotid artery; LS = left subclavian artery; W = wall.