Table 3.
Phenotypes of CRISPR Gene Knockdown F0 Zebrafish
| Target Gene | Reversed Heart Looping | Atrial and Ventricular Size | Circulation | Head/Brain/Eye Structures |
|---|---|---|---|---|
| cul3a | 8/64 (13) | Normal | Normal | Normal |
| wasf2 | 19/102 (19) | Normal | Normal | Normal |
| racgap1 | 6/105 (6) | Dilated atria and small ventricle 42/105 (42) | Normal | Hypoplastic 99/105 (95) |
| brk1 | 23/122 (19) | Normal | Normal | Normal |
| nckap1 | 16/83 (19) | Normal | Normal | Normal |
| cyfpl1 | 1/75 (1.3) | Normal | Normal | Normal |
| abi1 | 1/72 (1.4) | Normal | Normal | Normal |
Values are n/N (%). Most (5 of 7) candidate genes demonstrate abnormal cardiac development in CRISPR-mediated knockdown, including reversed cardiac looping in 3 WAVE2 complex genes (brk1, nckap1, and wasf2) and cul3a. Loss of racgap1 also demonstrated extracardiac anomalies in addition to a small ventricle and atrial dilation.
CRISPR = clustered regularly interspaced short palindromic repeats.