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. 2020 Mar 16;122(9):1342–1353. doi: 10.1038/s41416-020-0783-0

Fig. 5. The AKT-NF-κB signalling pathway is the crucial mechanism of CD133 regulating MDR1/P-gp expression in CRC.

Fig. 5

The AKT OE plasmid or the NF-κB/p65 OE plasmid could reverse the effects of CD133 KD plasmid in LoVo/ADR cells: a reversal of reduced P-gp expression in western blots; b reversal of reduced MDR1 mRNA expression by qPCR; c reversal of reduced MDR1 gene promoter activity by a luciferase reporter assay. d, e reversal of enhanced intracellular DOX concentrations; e reversal of reduced P-gp expression by immunofluorescence; f reversal of enhanced sensitivity in the CCK-8 assay; The AKT KD plasmid or the NF-κB/p65 KD plasmid could reverse the effects of CD133 OE plasmid in LoVo cells: a reversal of increased P-gp expression in western blots; b reversal of increased MDR1 mRNA expression by qPCR; c reversal of increased MDR1 gene promoter activity by a luciferase reporter assay. d, e reversal of reduced intracellular DOX concentrations; e reversal of increased P-gp expression by immunofluorescence; f reversal of reduced sensitivity in the CCK-8 assay; *p < 0.05, **p < 0.01. Each bar represents the mean ± SD of three independent experiments.