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. 2020 Apr 28;10:7153. doi: 10.1038/s41598-020-64060-x

Figure 1.

Figure 1

Biological model and data acquisition. (A) Frontal view of the Drosophila melanogaster olfactory system. Olfactory receptor neurons of the antennal nerve project to individual glomeruli of the antennal lobes, where they synapse with projection neurons and with local interneurons. From the antennal lobes, the olfactory information is conveyed by projection neurons to the mushroom body (MB), the olfactory learning and memory center, and to the lateral horn. Upon odor stimulation, a stereotypic pattern of glomeruli activity is observed in the antennal lobe, while the pattern of responses of Kenyon cells is not stereotypic because projections neurons are randomly connected to Kenyon cells in each fly. (B) Details of the dissection process, in which the MB is exposed for imaging. (C) Left: odor delivery system which simultaneously allows image acquisition and odor delivery to the fly. Middle: 3D volume rendering of a 3D stack after acquisition. Right: maximum intensity projections of nuclei and signal channels along Z axis (top) and Y axis (bottom) of a 3D stack. Scale bar is 10 µm.