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. 2020 Apr 28;10:7162. doi: 10.1038/s41598-020-64195-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Ramucirumab and/or Paclitaxel on cell migration and actin polymerization in gastric cancer cell lines. AGS, NCI-N87 [N87], HGC-27 and KATO III human gastric cancer cell lines were cultured for 48 h with 100 µg/ml Ram and/or 1 nM PTX. (a) The results obtained by migration assay performed on fibronectin coated wells is shown on the left. The percentage of migration were calculated at the time T0 and after 48 h (T2). The 100% represented the detection zone completely closed. The experiments were performed in triplicate and the mean values ± SD were plotted in the relative graph. *p < 0.05; **p < 0.001; ***p < 0.0001. The representative images of cell staining with DyLight 554 Phalloidin are presented on the right. Scale bar: 100 µm. (b) The expression of β-tubulin III (TUBB III) and EMT protein E-Cadherin and N-Cadherin after 48 h of Ram and/or PTX treatment was analyzed by Western Blotting in three independent experiments and a representative image is shown on the left. On the right are presented the data of three independent Real Time-PCR experiments of E-Cadherin plotted in the graphs and expressed as mean ± SD. *p < 0.05; ***p < 0.0001.