Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 28;11:2057. doi: 10.1038/s41467-020-15960-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Main steps include the decision of which lipids to query (Lipid Query), the calculation of the lipid precursor and corresponding fragment masses (Calculation) and the assay design (Targeted Assay). b These main steps consist of the selection of species or lipidomes to target (1–2), the inclusion of isotope-coded internal standards for validation (3), the calculation of precursor and fragment masses for the assay (4), library generation (5) and the in silico optimization of CE for individual fragments (6). The steps (1–6) are performed in LipidCreator. After the submission of the transition list to Skyline and the execution of the targeted lipidomics experiment, lipids can be validated by spectral library matching (7) and/or their coeluting internal standards, which are further used for quantification (8). Due to the integration features of Skyline, different downstream quality control systems, such as Panorama, are available (9). c Graphical user interface of LipidCreator integrated into Skyline.