Table 1.

General plasmids and primers.

Construct	Template (if not synthesized); amplified insert in bold; [reference]	5′-forward primer	5′-reverse primer	Linker
pcDNA3 β-arrestin2i1-Nluc	pcDNA3 β-arrestin2i1-Y2	GGTGGAAAGCTTATGGGGGAGAAACCCG	GCATCGATCCACCGCAGAGTTGATCATCATAGTC	*
	pAAVS1P-iCLHN Nluc (Addgene plasmid # 66579) (36)	CAATCGATCCACCGCTACCGCCACCGCCGGAACCGCCACCACCAGAACCGCCACCTCCGCCCGCCAGAATGCGTTC	GACCCAAGCTTGCCACCATGGTCTTCACACTCGAAGATTTCGTTGG
pcDNA3 β-arrestin1A-Nluc	β-arrestin1A RC201279 (Origine)	GGTGGAAAGCTTATGGGCGACAAAGGGACCCG	GCATCGATCCACCTCTGTTGTTGAGCTGTGGAGAGCC	*
pEYFP β-arrestin2-EYFP	Published in (37)	-	-
pcDNA3 hACKR4-EYFP	pcDNA3 CCR7-EYFP (9)	GGACTCGAGAGCGGAGGTGGCGGTTCTGGTGGTGGCGGTTCCGGCGGTGGCGGTAGCGTGAGCAAGGGCGAGGAG	GAATAGGGCCCTCTAGACTACTTGTACAGCTCGTCCATGC	**
pcDNA3 hACKR4t-EYFP	pcDNA3 ACKR4-EGFP (38)	GGAGACCCAAGCTTCATTACGATGGC	CTCTCGAGTCCACCAACGTACAAGATTGGGTTCAAACAAGAGTG	**
pcDNA3 hACKR4-mTq2	pcDNA3.1(-)Galphai1-mTurquoise2 (39)	GCAGACTCGAGAGCGGAGGTGGCGGTTCTGGTGGTGGCGGTTCCGGCGGTGGCGGTAGCATGGTGAGCAAGGGCGAGG	GCAGGTCTAGATTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCG	**
pcDNA3 hACKR4-HA	pcDNA3 ACKR4-EGFP (38)	GGAGACCCAAGCTTCATTACGATGGC	CTACCTCGAGCCCCAATAGAGAAGGTAGAAGT	***
pcDNA3 hCCR7-EYFP	pcDNA3 CCR7-HA (40)	GACCCAAGCTTGGTACCGAGCTCGGATC	GTAGCTCGAGTCCACCGGAGAAGGTGGTGGTGGTCTCG	**
pcDNA3 hCCR7-HA	Published in (40)	-	-	***
pSUMO hCCL19	pCR3-hCCL19-Fc (40)	GGTGCTCGAGTTAACTGCTGCGGCGCTTC	GACTAGGTCTCCGGTGGGGGCACCAATGATGCTGAAGACTG	-
pSUMO hCCL21	Published in (41)			-
pSUMO hCCL25	hCCL25 RC222128 (Origene)	GACTAGGTCTCCGGTGGGCAAGGTGTCTTTGAGGAC	GTGCTCGAGTTACAGTCCTGAATTAGCTGATATCAGGAGGG	-
pSUMO hCCL19-S6	pSUMO hCCL19	CCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGG	CAGGTGCTCGAGTTATTAGTTCAGCAGGCGCAGCAGCCAGCTCAGGCTATCGCCGCTGCCGCCGCCGCCGCTACTGCTGCGGCGCTTCATCTTGG	****

Linker sequence used between protein and tag.

^*GSI(GGGGS)₃.

^**GGLES(GGGGS)₃.

^***GARA.

^****SGGGGS.

^*****(GGGGS)₃GGS.