Table 2.

GRK related plasmids and primers.

Construct	Template (if not synthesized); amplified insert in bold; [reference]	5′-forward primer	5′-reverse primer	Linker
pRK5-hGRK4 missing XbaI site	pRK5-hGRK4 (addgene: #32690) (42)	GCTTTGCCATTAGATCTCGACAAGAACATACATAC	GTATGTA TGTTCTTGTCGAGATCTAATGGCAAAGC
pRK5-hGRK4 missing HindIII and XbaI site	pRK5-hGRK4 missing XbaI site	GTGAAAGTGAGGAAGCCTTGCCATTAGATCTCG	CGAGATCTAATGGCAAGGCTTCCTCACTTTCAC
pcDNA3 Nluc-GRK2	pWZL Neo Myr Flag ADRBK1(addgene: #20418) (43)	GTAGCGGTGGATCGATGGGGTCTTCAAAATCTAAACCAAAGGACC	GATAGGGCCCTCTAGATCAGAGGCCGTTGGCACTGCCGCGCTGGACCAGCGGCACCTTGCTCAGCTCCACCACGGGCGAG	*****
	pcDNA3 β-arrestin2i1-Nluc	GACCCAAGCTTGCCACCATGGTCTTCACACTCGAAGATTTCGTTGG	CAATCGATCCACCGCTACCGCCACCGCCGGAACCGCCACCACCAGAACCGCCACCTCCGCCCGCCAGAATGCGTTC
pcDNA3 GRK2-NLuc	pcDNA3 Nluc-GRK2	GAGACCCAAGCTTCATTACGATGGCGGACCTGGAG	GCAGCATCGATCCACCGAGGCCGTTGGCACTGC	*
pcDNA3 GRK3-Nluc	pDNR-Dual GRK3 (DNASU: HsCD00022400)	GAGACCCAAGCTTCATTACGATGGCGGACCTGGAGG	GCAGCATCGATCCACCGAGGCCGTTGCTGTTTCTGTG	*
pcDNA3 Nluc-Grk4	pRK5-hGRK4 missing HindIII and XbaI site	GTGGCGGTAGCGGTGGATCGATGGAGCTCGAGAACATCGTGGCCAAC	GAATAGGGCCCTCTAGATTAGCATTGCTTGGGTTCCACTTCCTTCTC	*****
pcDNA3 GRK5-Nluc	pWZL Neo Myr Flag GRK5 (addgene: #20495) (43)	GAGACCCAAGCTTCATTACGATGGAGCTGGAAAACATCGTG	GCAGCATCGATCCACCGCTGCTTCCGGTGGAGTTC	*
pcDNA3 GRK6-Nluc	Synthesized	CTATAGGGAGACCCAAGCTTATGGAGCTCGAGAACATCGTAGCG	CCTCCAATCGATCCACCCCGCCAACTGCTGGTGGGGGCCTCGGGCTG	*

Linker sequence used between protein and tag.

^*GSI(GGGGS)₃.

^*****(GGGGS)₃GGS.