Table 3.
Phosphorylation site mutations in ACKR4-EYFP.
| Mutation | Nucleotide mutation | 5′-forward primer | 5′-reverse primer |
|---|---|---|---|
| ACKR4Y68F | TA1103TT | GGTTGTTGCTATCTATGCTTTCTACAAGAAGCAAAG | CTTTGCTTCTTGTAGAAAGCATAGATAGCAACAACC |
| ACKR4Y79F | TA1136TT | GACCGATGTCTTCATTTTGAACTTGGCTGTTG | CAACAGCCAAGTTCAAAATGAAGACATCGGTC |
| ACKR4Y138F | TA1313TT | CATCTCTATTGATAGATTCGTTGCTGTTACCAAGG | CCTTGGTAACAGCAACGAATCTATCAATAGAGATG |
| ACKR4T142A | A1325G | GATACGTTGCTGTTGCCAAGGTCCCATCTC | GAGATGGGACCTTGGCAACAGCAACGTATC |
| ACKR4S146A | TCT1337GCC | CTGTTACCAAGGTCCCAGCCCAATCTGGTGTTGG | CCAACACCAGATTGGGCTGGGACCTTGGTAACAG |
| ACKR4S148A | TCT1343GCC | CAAGGTCCCATCTCAAGCCGGTGTTGGTAAACCATG | CATGGTTTACCAACACCGGCTTGAGATGGGACCTTG |
| ACKR4T226A | ACT1577GCC | GCTACTTCATTACCGCTAGAGCCTTGATGAAGATGCCAAACATC | GATGTTTGGCATCTTCATCAAGGCTCTAGCGGTAATGAAGTAGC |
| ACKR4S236A | T1607G | CAAACATCAAGATCGCCAGACCATTGAAGG | CCTTCAATGGTCTGGCGATCTTGATGTTTG |
| ACKR4S309A | TCT1826GCC | CGTTTTTATGGGTGCCGCCTTCAAGAACTACG | CGTAGTTCTTGAAGGCGGCACCCATAAAAACG |
| ACKR4S323A | TCT1868GCC | GCTAAGAAGTACGGTGCCTGGAGAAGACAAAGACAATC | GATTGTCTTTGTCTTCTCCAGGCACCGTACTTCTTAGC |
| ACKR4S330A | T1889G | GAAGACAAAGACAAGCCGTTGAAGAATTCCC | GGGAATTCTTCAACGGCTTGTCTTTGTCTTC |