. 2020 Apr 22;11:720. doi: 10.3389/fimmu.2020.00720

Table 4.

Templates for Gα and site directed mutations thereof.

Gα variant	Mutation effect	5′-forward primer	5′-reverse primer
Gα_i/o/q-RLuc8	A kind gift from Nevin Lambert (44)
Gα_s	Synthesized
Gα₁₃	A kind gift from B.Moepps. (45)
pIRES Gß-2A-cpV-Gy2 GNAi3-mTq2	addgene #69625 (39)
Gαo	– BamHI site	CGCAAGAAGTGGATTCATTGCTTCGAGGAC	GTCCTCGAAGCAATGAATCCACTTCTTGCG
GαS	– BamHI site	CATTGTGAAGCAGATGAGAATCCTGCATGTTAATGG	CCATTAACATGCAGGATTCTCATCTGCTTCACAATG
	– BamHI site	GCCGCAAGTGGATACAGTGCTTCAACG	CGTTGAAGCACTGTATCCACTTGCGGC
	+ BamHI site	CCCCCCGTGGAGCTGTCAGGTGGCGGATCCCAGTTCAGAGTGG	CCACTCTGAACTGGGATCCGCCACCTGACAGCTCCACGGGGGG
Gα13	– BamHI site	CATATTCCCTGGTCAGGTGGCGGATCCGGAGACAACTC	GAGTTGTCTCCGGATCCGCCACCTGACCAGGGAATATG
	– HindIII site	CTCGAGAGAAGCTCCATATTCCCTGGG	CCCAGGGAATATGGAGCTTCTCTCGAG
	+ BamHI site	CTATTTCCTAGAATTTGAAGGCGATCCCCACTGCTTAAGAGAC	GTCTCTTAAGCAGTGGGGATCGCCTTCAAATTCTAGGAAATAG
pIRES	– HindIII site	AATGTCGTGAAGGAAGCAGTACCTCTGGTAGCTTCTTGAAGACAAACAAC	TTGTTTGTCTTCAAGAAGCTACCAGAGGTACTGCTTCCTTCACGACATTC
	+ HindIII site	GTTTTCCTTTGAAAAACACGATGATAATAAGCTTTGCACGTTGAGCGCCGAAGACAAGGCGG	CCGCCTTGTCTTCGGCGCTCAACGTGCAAAGCTTATTATCATCGTGTTTTTCAAAGGAAAAC