. 2020 Apr 22;11:720. doi: 10.3389/fimmu.2020.00720

Table 5.

Templates for Gα and Nluc amplification.

Affected protein	Amplification of	5′-forward primer	5′-reverse primer
NLuc	BamHI-Nluc-BamHI	TCAGGTGGCGGATCCATGGTCTTCACACTCGAAGATTTCGTTG	GATGCCGGATCCTCCACCGCCAGAGCCCGCCAGAATGCGTTCGCAC
Gα_o	HindIII-Gαo(1)-BamHI	GAGACCCAAGCTTCAGCCACCATGGGATGTACTCTGAGCGCAGAGGAG	GGATCCGCCACCTGACAAAGTGTCCATGGCCCGGACGATGGCTGCCAGGGAC
	BamHI-Gα o(2)-XbaI	TCTGGCGGTGGAGGATCCGGCATCGAATATGGTGATAAGGAGAGAAAG	CAGGGCCCTCTAGATCAGTACAAGCCGCCGCCCCGGAG
Gα_i	HindIII-Gαi(1)-BamHI	GGATCCGCCACCTGACAACCTCCCCATAGCCCTAATGATAGCAATAATTGACTG	GAGACCCAAGCTTCAGCCACCATGGGCTGCACGCTGAGC
	BamHI-Gαi(2)-XbaI	TCTGGCGGTGGAGGATCCAAGATAGACTTTGGTGACTCAGCCCG	GTATGCCTCTAGATCAAAAGAGACCACAATCTTTTAGATTA
Gα₁₃	HindIII-Gα₁₃-XbaI	GACCCAAGCTTATGGCGGACTTCCTGCCGTC	CAGGGCCCTCTAGATTACTGTAGCATAAGCTGCTTGAGGTTGTC
Gα_S	HindIII-Gα_S-XbaI	GACCCAAGCTTATGGGCTGCCTC	CAGGGCCCTCTAGATTAGAGCAGCTCGTACTGACGAAGGTG
IRES sequence	amplification	GTTCGAAGTCGACAGATCTC	CATCGCTCTAGACGTACTAGCAAGCTTATTATCATCGTGTTTTTCAAAGG