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. 2020 Apr 22;8:267. doi: 10.3389/fcell.2020.00267

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Copyright © 2020 Yoshimoto, Ikemoto-Uezumi, Hitachi, Fukada and Uezumi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Re-expression of Myoz1 protein is closely associated with the extent of myofiber regeneration. (A) TA muscle sections from the indicated time points were subjected to immunofluorescent staining for Myoz1 (green) and Laminin α2 (magenta) followed by HE staining. (B) Area occupied by Myoz1+ fibers in intact muscle (Day 0) and Myoz1+ centrally nucleated fibers in regenerating muscle was quantified. Data represent the mean ± SD of n = 6 mice at each time point. Data from regenerating muscle (Day 3 to Day 14) were analyzed by ANOVA followed by Tukey’s test to evaluate statistical difference. (C) Magnified images of boxed areas in (A). Upper panels show area with advanced regeneration and lower panels show area with delayed regeneration. Scale bars: 100 μm (A) and 20 μm (C).