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. 2020 Apr 22;11:729. doi: 10.3389/fmicb.2020.00729

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Copyright © 2020 Zhan, Song, Wang, Guo, Shen, Hua, Shi, Angel, Gu, Xie, Lai, Peng and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolocalization and Transcription levels of Eg-IAP and Eg-BIRP in E. granulosus. (A) Fluorescence immunohistochemistry analysis of Eg-IAP and Eg-BIRP at different stages of E. granulosus life cycle. rEg-IAP/rEg-BIRP were localized on the protoscolex, fertile/infertile cyst walls, and 18-day strobilated worm using specific anti-rEg-IAP/anti-rEg-BIRP rabbit sera IgG or pre-immune rabbit sera IgG. Abbreviations: H, hooks; LL, laminated layer; GL, germinal layer; Teg, tegument; PR, parenchymal region. Note, Fluorescence-labeled regions were marked with arrows. (B) qRT-PCR analysis of transcription levels of Eg-IAP and Eg-BIRP at PSCs and 18-day strobilated worms stages. Data are presented as the means ± SD (n = 3). Statistically significant differences between PSCs and 18-day strobilated worms were determined using Student’s t-tests (*p < 0.05).