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. 2020 Apr 22;11:729. doi: 10.3389/fmicb.2020.00729

FIGURE 3.

FIGURE 3

In vitro activity of LCL161 against PSCs. (A) Survival rate in PSCs incubated with LCL161 for 8 h, and the viability of the PSCs was assessed by Trypan blue staining. Data are presented as the means ± SD (n = 3). Statistically significant differences between blank control group and three dosing groups (*p < 0.05), and no significant differences between blank control group and negative control group (p > 0.05) were determined using Student’s t-tests. Structural alterations (B) and ultrastructural alterations (C) in PSCs incubated with LCL161 under light microscope and TEM, respectively. (a) PSCs of 200 μmol/L LCL161 group; (b) PSCs of 400 μmol/L LCL161 group; (c) PSCs of 800 μmol/L LCL161 group; (d) PSCs of blank control group; (e) PSCs of negative control group. Abbreviations: B, bilayer; H, hooks; CC, calcareus corpuscles; R, rostellar region; S, sucker; SR, soma region; N, nucleus. Note:Red arrows indicate typical morphological changes associated with apoptosis and black arrows indicate the different structural characteristics of PSCs.