FIGURE 3.

Decreased percentages and impaired suppressive factors level in MDSC of arg^myeKO mouse during colitis. WT and Arg^myeKO mice treated with 3.5% DSS for 9 days. Mice were sacrificed and PBMC, splenic cells and LPMC were isolated to detect MDSC as indicated. Flow cytometry of MDSC showing the MDSC in PBMC (A,B), spleen (C,D), LPMC (E), (F) qPCR analysis of Arg-1, iNOS and IDO in the colorectum of WT and arg^myeKO mice. *P < 0.05. Data are representative of three independent experiments with similar results. Quantification of signal was shown in bar graphs and error bars represent mean ± SD.