FIGURE 7.
Quercetin enhanced suppressive factors expression in MDSC by improved pSTAT3 levels after binding to ESR. WT mice treated with 3.5% DSS for 9 days. Mice were sacrificed and qPCR analysis of ESR1, ESR2 (A), Arg-1 and iNOS (G) of the colorectum in WT and argmyeKO mouse during colitis. Flow cytometry of pSTAT3 of MDSC in PBMC (B–D) and spleen (E,F). Flow cytometry of Arg-1 in MDSC (G) and subpopulations (H,I) of PBMC; Flow cytometry of Arg-1 in MDSC (J) and subpopulations (K,L) of spleen, gray, isotype; dotted, vehicle treatment; solid, quercetin treatment. *P < 0.05; **P < 0.01; ***P < 0.001. Data are representative of three independent experiments with similar results. Quantification of signal was shown in bar graphs and error bars represent mean ± SD.