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. 2020 Apr 22;11:687. doi: 10.3389/fimmu.2020.00687

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Copyright © 2020 Ma, Zhen, Hu and Yi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MDSC-derived Arg-1 enhanced IL-17A, but decreased IL-17F expression in TH17 cells WT mice treated with 3.5% DSS for 9 days. Mice were sacrificed and qPCR analysis of IL-17A (A) and IL-17F (B) in the colorectum of WT and arg^myeKO mouse during colitis. Before IL-17A/F detection, cells were stimulated with cocktail for 6 h. Flow cytometry of TH17 cells in PP (C,D) and mLN (E,F). Flow cytometry of γδT cells in PP (G–I). *P < 0.05; **P < 0.01; ***P < 0.001. Data are representative of three independent experiments with similar results. Quantification of signal was shown in bar graphs and error bars represent mean ± SD.