FIGURE 2.

qPCR plate setup and analysis settings. (A) Plate setup view for the Bio-Rad CFX Manager v3.1 software showing correct name convention: orange box – one primer kit set for five DNA samples; red box – sample name is the same for both wells; green box – target name and allele name are separated by the underscore sign; blue box – control gene name is the same for all wells; (B) The AScall import and preprocessing options, no amplification range, curve fitting model, Cq determination method can be set for preprocessing.