TABLE 1.

Quality check codes description.

QC name	Message
2*RFU_QC	“OK”
	“Low” – curve endpoint fluorescence signal is lower than “RFU Threshold”
2*ampStatus_QC	“OK”
	“NoAmp” – amplification for this curve is not detected
2*replicateMatch_QC	“OK”
	“Fail” – Cq difference between replicates bigger than “Cq Δ” option
2*noAmpNTC_QC	“OK”
	“Fail” – any NTC sample has positive amplification
2*ctrlMarker_QC	“OK”
	“Fail” – control marker does not have amplification in a well
2*allelesDeltaCq_QC	“OK”
	“Fail” – Cq difference between alleles of a marker is bigger than “Cq Δ” option