FIG 4.

MCM1 overexpression activates transcription of ECE1. (A) The wild type and the ahr1Δ mutant with integrated pECE1-GFP and with or without pADH1-MCM1 were grown in SDG medium with or without 10% human serum for 6 h at 37°C prior to microscopy. Scale bar, 20 μm. (B and D) Total RNA from the wild-type and ahr1Δ mutant strains (B) and from the wild-type and tup1Δ and cph1Δ/efg1Δ mutant strains was isolated after 6 h growth and used to determine the relative ECE1 expression levels. Scale bars, 20 μm. Asterisks indicate significant changes (P ≤ 0.05, two-tailed, unpaired Student's t test) compared to the corresponding strain without MCM1 overexpression. (C) The indicated strains were grown for 6 h at 37°C in SDG medium with or without 10% human serum prior to microscopy. Scale bar, 10 μm.