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. 2020 Apr 28;11(2):e00218-20. doi: 10.1128/mBio.00218-20

TABLE 1.

Methods and resources for studying the urinary microbiomea

Topic Methods Referencesb
Study design Cross sectional 10, 21, 53, 137139
Longitudinal 2, 44, 53, 103, 137, 138
Sample collection Voided/midstream clean catch 5, 9, 16, 17, 2732, 67
Transurethral catheterization 4, 9, 10, 13, 16, 18, 19, 22, 24, 31, 67
Suprapubic aspiration 9, 67
Data and metadata acquisition Culture based 4, 13, 19
16S rRNA amplicon sequencing 4, 5, 9, 10, 1318, 21, 22, 24, 2633, 67
Whole-genome metagenomics 11, 13, 15, 25, 53, 67
Data analysis Taxonomic profiling 4, 5, 9, 10, 1318, 21, 22, 24, 2633, 140
Functional analysis 11, 13, 15, 25, 53
Meta-analysis 53, 134, 135, 138, 141, 142
Data democratization Data curation, structure, public availability and ethics 53, 134, 142144
a

The table presents a summary of selected topics, methods, and references relevant to studying the human UT and UGT microbiome.

b

References 53, 67, 134, 137 to 139, and 140 to 144 consist of relevant technical reviews.