FIG 7.

Overproduction of ZapT causes aberrant localization of FtsZ and MipZ. The images show localization of FtsZ, the terminus region, MipZ, and the nucleoid in the presence of excess ZapT. The pQF or pQF::zapT vector was introduced into a strain expressing FtsZ-YFP (SHQ67) (A), FROS-ter (SHQ54) (B), MipZ-YFP (SHQ66) (C), or none of those products (NA1000) (D). Log-phase cells that were incubated for 6 h in PYE medium supplemented with 1 μM cumate were analyzed by fluorescence microscopy. (D) For visualization of nucleoid, cells fixed in 70% ethanol were incubated for >10 min in 2 μg/ml DAPI solution before imaging. Arrowheads indicate polar MipZ-free zones.