TABLE 1.
Bacterial strains used in this study
| Species and strain | Genotype | Reference or source |
|---|---|---|
| Caulobacter crescentus | ||
| NA1000 | A wild-type Caulobacter crescentus strain | 61 |
| PV2865 | CB15N cc0006::(tetO)n xylX::pHPV472 podJ::pHPV489 | 45 |
| SHQ10 | NA1000 CCNA_01434-3xFLAG | This study |
| SHQ48 | NA1000 ΔzapT | This study |
| SHQ56 | NA1000 zapA::mCherry | This study |
| SHQ63 | SHQ56 xylX::pHPV472 podJ::pHPV489 | This study |
| SHQ66 | NA1000 mipZ::pMT151(PmipZ::mipZyfp Pxyl::mipZ) | This study |
| SHQ67 | NA1000 vanA::pMT383(ftsZyfp) | This study |
| SHQ68 | NA1000 ΔCCNA_03356 (zapA) | This study |
| SHQ69 | NA1000 ΔCCNA_03357 (zauP) | This study |
| SHQ75 | SHQ48 zapA::mCherry xylX::pHPV472 podJ::pHPV489 | This study |
| SHQ136 | SHQ48 vanA::pMT383 (ftsZyfp) | This study |
| SHQ143 | NA1000 zapT::mNeonGreen | This study |
| SHQ173 | SHQ143 zapA::mCherry | This study |
| SHQ177 | SHQ143 lacA::Pcumate-lacImCherry | This study |
| SHQ178 | SHQ177 podJ::pHPV489 | This study |
| UJ9492 | NA1000 3xFLAG-zauP | This study |
| UJ9812 | NA1000 zapA-3xFLAG | This study |
| Escherichia coli | ||
| DH5a | A general cloning strain | Invitrogen |
| Top10 | A general cloning strain | Invitrogen |
| UJ5191 | S17-1 pMT151 | 42 |
| UJ5195 | S17-1 pMT383 | 42 |
| UJ9399 | DH10B pNPTS-ZapA-mCh | This study |
| UJ9486 | DH10B pNPTS-3FzapB | This study |
| UJ9524 | Top10 pQF | 65 |
| UJ9727 | DH10B pNPTS138-01434_3F | This study |
| UJ9811 | Top10 pNPTSzapA3F | This study |
| UJ10015 | Top10 pNPTSzapA-CKO | This study |
| UJ10016 | Top10 pNPTSzapB-CKO | This study |
| UJ10032 | Top10 pQF01434-3F | This study |