TABLE 2.
Plasmids used in this studya
| Plasmid | Description | Reference or source |
|---|---|---|
| mNG-sfTq2 | A plasmid with the mNeonGreen gene | Addgene |
| pET21a01434_3F6H | A pET21a (Novagen) derivative to purify ZapT with C-terminal 3xFLAG and 6×His | This study |
| pLacQF | A pNPTS138 derivative carrying the cumate-dependent promoter between the upstream and downstream regions of the lacA gene |
This study |
| pLacQFlacImCherry | A pLacQF derivative with the lacImCherry gene | This study |
| pMT151 | A kanamycin resistance-integrating plasmid with the MipZ-YFP ORF under the control of the xylose-dependent promoter |
42 |
| pMT383 | A kanamycin resistance-integrating plasmid with the FtsZ-YFP ORF under the control of the vanillate-dependent promoter |
42 |
| pNPTS-3FzapB | A pNPTS138 derivative with 3F-zapP | This study |
| pNPTS-ZapA-mCh | A pNPTS138 derivative with zapA-mCherry | This study |
| pNPTS01434-CKO | A pNPTS138 derivative to introduce an in-frame deletion of zapT | This study |
| pNPTS01434-mNG | A pNPTS138 derivative with zapT-mNeonGreen | This study |
| pNPTS138 | A kanamycin-resistant suicide vector | 66 |
| pNPTS138-01434_3F | A pNPTS138 derivative with ZapT-3F | This study |
| pNPTSzapA-CKO | A pNPTS138 derivative to introduce an in-frame deletion of zapA | This study |
| pNPTSzapA3F | A pNPTS138 derivative with ZapA-3F | This study |
| pNPTSzapB-CKO | A pNPTS138 derivative to introduce an in-frame deletion of zauP | This study |
| pQF | A low-copy-number tetracycline-resistance-conferring vector with the cumate-dependent promoter | 65 |
| pQF01434-3F | A pQF derivative with zapT-3F | This study |
| pQFzapTmNG | A pQF derivative with zapT-mNeonGreen | This study |
| pRVCHYC-2 | A kanamycin-resistance-conferring plasmid with the mCherry ORF | 47 |
a
ORF, open reading frame.