TABLE 2.
Specific enzyme activities of purified PedE and PedH with the four tested growth substrates at 10 mM measured with 2,6-dichlorophenolindophenol (DCPIP)-dependent colorimetric assay
Substrate | Mean specific activity (U mg−1) ± SDa |
|
---|---|---|
PedE (1 mM Ca2+) | PedH (1 μM La3+) | |
Citrate | n.d.b | n.d.b |
Glucose | n.d.b | n.d.b |
Glycerol | 0.3 ± 0.1 | 0.9 ± 0.1 |
2-Phenylethanol | 8.0 ± 0.4 | 6.3 ± 0.3 |
Data represent averages of results from biological triplicates with standard deviations (SD).
Activities below detection limit are indicated (n.d.).