TABLE 3.
Lag times (λ), maximal OD600 during stationary phase (OD600max), and maximal growth rates (μmax) of different P. putida strains during growth on M9 medium supplemented with 20 mM glycerol and 0 μM or 10 μM La3+ incubated in microtiter plates at 30°C and 250 rpm (see also Fig. 2 and Fig. 5)
Strain | λ [h] ± SD |
OD600max ± SD |
μmax [h−1] ± SD |
|||
---|---|---|---|---|---|---|
0 μM La3+ | 10 μM La3+ | 0 μM La3+ | 10 μM La3+ | 0 μM La3+ | 10 μM La3+ | |
P. putida KT2440* | 18.4 ± 0.3 | 9.8 ± 0.2 | 0.894 ± 0.007 | 0.771 ± 0.020 | 0.351 ± 0.008 | 0.277 ± 0.007 |
P. putida ΔpedE | 21.4 ± 0.4 | 10.5 ± 0.1 | 0.916 ± 0.007 | 0.774 ± 0.026 | 0.299 ± 0.008 | 0.289 ± 0.006 |
P. putida ΔpedH | 17.9 ± 0.3 | 21.6 ± 0.5 | 0.849 ± 0.012 | 0.887 ± 0.001 | 0.352 ± 0.028 | 0.292 ± 0.006 |
P. putida ΔpedE ΔpedH | 21.8 ± 0.1 | 21.0 ± 0.2 | 0.887 ± 0.001 | 0.880 ± 0.066 | 0.305 ± 0.006 | 0.315 ± 0.007 |
P. putida ΔgarK | 21.4 ± 0.1 | n.d.a | 0.858 ± 0.005 | 0.042 ± 0.002 | 0.322 ± 0.004 | n.d.a |
P. putida ΔcalA | 18.7 ± 0.5 | 10.6 ± 0.1 | 0.891 ± 0.001 | 0.783 ± 0.009 | 0.350 ± 0.013 | 0.307 ± 0.006 |
P. putida ΔglcDEF | 19.6 ± 0.1 | 11.0 ± 0.1 | 0.885 ± 0.003 | 0.775 ± 0.006 | 0.335 ± 0.003 | 0.287 ± 0.001 |
No growth parameters were determined for cultures that did not reach stationary phase during incubation (n.d.).