Skip to main content
. 2020 Apr 28;11(2):e00516-20. doi: 10.1128/mBio.00516-20

TABLE 3.

Lag times (λ), maximal OD600 during stationary phase (OD600max), and maximal growth rates (μmax) of different P. putida strains during growth on M9 medium supplemented with 20 mM glycerol and 0 μM or 10 μM La3+ incubated in microtiter plates at 30°C and 250 rpm (see also Fig. 2 and Fig. 5)

Strain λ [h] ± SD
OD600max ± SD
μmax [h−1] ± SD
0 μM La3+ 10 μM La3+ 0 μM La3+ 10 μM La3+ 0 μM La3+ 10 μM La3+
P. putida KT2440* 18.4 ± 0.3 9.8 ± 0.2 0.894 ± 0.007 0.771 ± 0.020 0.351 ± 0.008 0.277 ± 0.007
P. putida ΔpedE 21.4 ± 0.4 10.5 ± 0.1 0.916 ± 0.007 0.774 ± 0.026 0.299 ± 0.008 0.289 ± 0.006
P. putida ΔpedH 17.9 ± 0.3 21.6 ± 0.5 0.849 ± 0.012 0.887 ± 0.001 0.352 ± 0.028 0.292 ± 0.006
P. putida ΔpedE ΔpedH 21.8 ± 0.1 21.0 ± 0.2 0.887 ± 0.001 0.880 ± 0.066 0.305 ± 0.006 0.315 ± 0.007
P. putida ΔgarK 21.4 ± 0.1 n.d.a 0.858 ± 0.005 0.042 ± 0.002 0.322 ± 0.004 n.d.a
P. putida ΔcalA 18.7 ± 0.5 10.6 ± 0.1 0.891 ± 0.001 0.783 ± 0.009 0.350 ± 0.013 0.307 ± 0.006
P. putida ΔglcDEF 19.6 ± 0.1 11.0 ± 0.1 0.885 ± 0.003 0.775 ± 0.006 0.335 ± 0.003 0.287 ± 0.001
a

No growth parameters were determined for cultures that did not reach stationary phase during incubation (n.d.).