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. 2020 Apr 22;11:741. doi: 10.3389/fimmu.2020.00741

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Copyright © 2020 Junqueira-Kipnis, Trentini, Marques Neto and Kipnis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of neutrophils in draining secondary lymphoid organs. C57BL/6 mice (N = 5) were immunized with saline, BCG (10⁷ CFU/mL) or mc²-CMX (10⁸ CFU/mL), and after 2, 5, and 7 days, axillary lymph nodes (A,C) and spleen (B,D) were collected and processed to evaluate the number of neutrophil (A,B) and its activation status (C,D). *Statistical difference between vaccinated and saline groups (p < 0.05). ^#Statistical difference between BCG and mc²-CMX groups (p < 0.05).