Figure 3.

NK cells induced by the mc²-CMX and BCG vaccines. C57BL/6 mice were immunized with saline, BCG (10⁷ CFU/mL) or mc²-CMX (10⁸ CFU/mL) after 2, 5, and 7 days, the inflammatory lesions generated at the vaccination sites (B), the axillary lymph nodes (C) and spleen (D) were collected and processed to evaluate the NK cells. (A) Representative gates evaluating NK cells (spleen), 7 days after immunization. (B) Percentage of NK cells located at the vaccination site. (C) Total NK cells in axillary lymph nodes. (D) Total NK cells in the spleen. *Statistical difference between vaccinated and the saline groups (p < 0.05). ^#Statistical difference between BCG and mc²-CMX groups (p < 0.05).