Figure 6.

NK cell depletion. C57BL/6 mice were treated with anti-asialo antibody and after the first vaccination at 1- or 5-days post depletion the spleens were collected and checked for NK cell depletion (A). Representative flow cytometry showing depletion induced by the anti-Asialo GM1 antibody in the spleen (B). (C–E) C57BL/6 mice (N = 5) after NK cell depletion were immunized with saline, BCG (10⁷ CFU/mL), mc² (10⁸ CFU/mL), or mc²-CMX (10⁸ CFU/mL). After 15 days of the last vaccination with mc²-CMX, the mice were euthanized, and axillary lymph nodes, spleen, and lungs were collected for evaluation of the presence of neutrophils (Gr1⁺ cells) by flow cytometry. (A) Total neutrophils present in the axillary lymph nodes. (B) Total neutrophils present in the spleen. (C) Total neutrophils present in the lung. *Statistical difference between untreated groups and vaccinated groups. ^#Statistical difference between groups and mc²-CMX vaccinated groups (p < 0.05).