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. 2020 Mar 10;12(2):668–678. doi: 10.1111/os.12568

Figure 3.

Figure 3

The biological function of the 0404 cell line was inhibited after transfection of the RUNX2 plasmids. (A, F) Experimental and statistical results of the EdU assay after the transfection of CV and RUNX2 plasmids; (B, G) Experimental and statistical results of the scratching assay after the transfection of CV and RUNX2 plasmids. The first row is the photo taken just after the scratching, and the second row is the photo taken after 24h; (C, H) Experimental and statistical results of the transwell invasion assay. The time to pass through Matrigel is about 36h; (D, I) Experimental and statistical results of the apoptosis assay. 0404 cells were stained by FITV Annexin V / PI approximately 24 h after transfection, and detected by flow cytometry. (E) Experimental and statistical results of CCK8 assay. “*” and “***” indicate P < 0.05 and P < 0.001, respectively.