FIG 2.

Brain-specific loss of long-chain fatty acid oxidation does not systemically affect peripheral metabolism in CPT2^B−/− mice. (A) Body weights of male and female CPT2^lox/lox and CPT2^B−/− mice from the beginning of low-fat or high-fat diets at 3 weeks of age to 18 weeks of age. Males, CPT2^lox/lox, low-fat, n = 16, and high-fat, n = 14; CPT2^B−/−, low-fat, n = 8, and high-fat, n = 17. Females, CPT2^lox/lox, low-fat, n = 9, and high-fat, n = 14; CPT2^B−/−, low-fat, n = 3, and high-fat, n = 13. The data were analyzed using Student two-tailed t tests. (B) Blood glucose in 18-week-old male CPT2^lox/lox and CPT2^B−/− mice after 15 weeks of low-fat and high-fat diets. CPT2^lox/lox, low fat, n = 16, and high fat, n = 14; CPT2^B−/−, low fat, n = 8, and high fat, n = 17. (C) Serum metabolites (β-hydroxybutyrate, NEFAs, and triacylglycerol) in 18-week-old male CPT2^lox/lox and CPT2^B−/− mice after 15 weeks of low-fat and high-fat diets. CPT2^lox/lox, low fat, n = 8, and high fat, n = 8; CPT2^B−/−, low fat, n = 8, and high fat, n = 8. The data were analyzed using ordinary two-way analysis of variance with Sidak’s test for multiple comparisons. The data are expressed as means ± SEM. *, α = 0.05; ***, α = 0.001.