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. 2020 Apr 28;40(10):e00037-20. doi: 10.1128/MCB.00037-20

FIG 3.

FIG 3

Loss of brain-specific long-chain fatty acid oxidation results in a subtle impact on animal behavior. (A to D) Three-zone open-field test. (A) Key for operationalization of inner, middle, and outer zones in 3-zone open-field test. Shown are representative path traces from adult female CPT2lox/lox and CPT2B−/− mice depicting normal movement and thigmotaxis. (B) Total number of times the inner, middle, and outer zones were entered by adult female CPT2lox/lox and CPT2B−/− mice (n = 10). Shown are total percentages of time spent in the inner, middle, and outer zones by adult female CPT2lox/lox and CPT2B−/− mice (n = 10). The data were analyzed using Student two-tailed t tests. (C) Total distances traveled in inner, middle, and outer zones by adult female CPT2lox/lox and CPT2B−/− mice (n = 10). The data were analyzed using Student two-tailed t tests. (D) Total numbers of immobile episodes, grooming episodes, and rearing episodes in adult female CPT2lox/lox and CPT2B−/− mice (n = 10). The data were analyzed using Student two-tailed t tests. (E) Three-trial social recognition test. Shown are the total amounts of time adult female CPT2lox/lox and CPT2B−/− mice spent investigating a pup initially (Trial 1; n = 8) and in a subsequent investigation (Trial 2; n = 8) and time spent investigating a novel pup after the first two trials (Novel Mouse; n = 8). The data were analyzed using repeated-measures two-way analysis of variance with Sidak tests for multiple comparisons. (F) Rotarod test. Shown is latency to fall in rotarod tests performed on adult male and female CPT2lox/lox and CPT2B−/− mice on LFD and HFD. Males, CPT2lox/lox, low fat (n = 16) and high fat (n = 12); CPT2B−/−, low fat (n = 8) and high fat (n = 17). Females, CPT2lox/lox, low fat (n = 9) and high fat (n = 11); CPT2B−/−, low fat (n = 3) and high fat (n = 15). The data were analyzed using ordinary two-way analysis of variance with Sidak tests for multiple comparisons. The data are expressed as means and SEM. *, α = 0.05; **, α = 0.01; ns, not significant.