FIG 6.

Long-chain acylcarnitines are contained within the CNS and are unaffected by diet. (A and C) Concentrations of steady-state acylcarnitines were determined using approximately 25 mg of cortex tissue from 9-week-old CPT2^lox/lox and CPT2^B−/− mice given the control fed diet and after a 24-h fast (n = 6). (B and D) Additionally, steady-state acylcarnitines were determined using approximately 25 mg of cortex tissue from 18-week-old CPT2^lox/lox and CPT2^B−/− mice after 15 weeks on a low-fat or high-fat diet (n = 6). (E) Representative heat map images of charged mass acylcarnitine species and a phosphatidylcholine (PC K+) control from 50-μm-resolution MALDI IMS of 10-μm coronal brain slices from 24-h-fasted 6-month-old CPT2^lox/lox (n = 3) and CPT2^B−/− (n = 3) mice. The data are expressed as means and SEM. The data were analyzed using ordinary and repeated-measures two-way analysis of variance where appropriate, with Sidak tests for multiple comparisons. *, α = 0.05; **, α = 0.01; ***, α = 0.001; ****, α = 0.0001.