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. 2020 Apr 28;11:2054. doi: 10.1038/s41467-020-15937-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a UMAP plots of CyTOF data comparing CD45⁺HLA-DR⁺ cells generated using MS5_FS12 stromal cells either in vitro or in vivo. Relative expression of selected markers is shown for each condition. b Relative expression of selected markers highlighting the phenotypic differences between cDC2s generated in vitro and in vivo using MS5_FS12 stromal cells. c Heatmap displaying gene expression of the top ten genes of blood pDC and AS-DC published signatures²⁰ in pDC and pre/AS-DC generated in vitro, in vivo, and isolated from blood PBMC (n = 2 independent donors for pDC and pre/AS-DC generated in vivo and n = 3 independent donors for pDC and pre/AS-DC generated in vitro or isolated from peripheral blood). d Heatmap displaying gene expression of the blood cDC2 published signature²⁰ in cDC2 cells generated in vitro, in vivo and isolated from blood PBMC (n = 3 independent donors). e Volcano plot showing differentially expressed genes between in vitro and in vivo generated cDC2 (Log2FC > 1.5, adjusted p-value < 0.05). Statistical significance was calculated using Wald test with a Benjamin–Hochberg p-value correction (n = 3 independent donors per group). f Heatmap displaying gene expression of activation markers and co-stimulatory molecules expressed in cDC2 generated in vivo and in vitro (n = 3 independent donors).