Table 1.
Cryo-EM and refinement statistics for the PFV2.
| Parameter | PFV2 |
|---|---|
| Data collection and processing | |
| Magnification | 59,000× |
| Voltage (kV) | 300 |
| Electron exposure (e− Å−2) | 20 |
| Defocus range (μm) | −1.25 to −2.25 |
| Pixel size (Å) | 1.4 |
| Symmetry imposed | Helical (rise: 2.86 Å; rotation: 22.95°) |
| Final particle images (n) | 186,576 |
| Map resolution (Å) | |
| ‘Gold-standard’ map: map FSC (0.143) | 3.4 |
| Model: map FSC (0.38, which is √0.143) | 3.4 |
| d99 | 3.5 |
| Refinement | |
| Initial model used (PDB code) | NA |
| Map-sharpening B factor (Å2) | −155 |
| Root-mean-square deviations | |
| Bond lengths (Å) | 0.008 |
| Bond angles (°) | 0.828 |
| Validation | |
| MolProbity score | 1.37 |
| Clash score | 4.0 |
| Poor rotamers (%) | 0 |
| Ramachandran plot | |
| Favored (%) | 96.9 |
| Allowed (%) | 3.1 |
| Disallowed (%) | 0 |
The PFV2 model used has the following IDs: Electron Microscopy Data Bank: 21094; PDB: 6V7B.