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. 2020 Apr 28;40(4):BSR20192761. doi: 10.1042/BSR20192761

Figure 3. Knockdown of CD47 cooperated with H2 to repress the progression of lung cancer.

Figure 3

A549 and H1975 cells transfected with si-CD47 or si-NC were treated with 60% H2, then the following assays were performed. (A) Western blotting assay was used to detect the knockdown efficiency of si-CD47 in A549 and H1975 cells. (B) CCK-8 assay was used to detect cell proliferation. (C) Transwell chambers were applied for cell invasion assessment. (D,E) Wound healing assay was used to detect cell migration ability. (F,G) The effects of H2 treatment on the apoptosis of A549 and H1975 cells were determined by flow cytometry assay. (H) Western blotting was carried out to detect the expressions of CD47, CDC42, Bcl-2, cleaved caspase3, caspase3, cleaved PARP and PARP after 48 h of cell treatment with 60% H2 (*P<0.05, **P<0.01 vs. control group; #P<0.05, ##P<0.01 vs. H2 group).