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. Author manuscript; available in PMC: 2020 Apr 29.
Published in final edited form as: Nat Protoc. 2019 Jul 24;14(8):2571–2594. doi: 10.1038/s41596-019-0195-x
 Component Volume for 96-well plate with overage (μL) Volume for 384-well plate with overage (μL) Final concentration
NEBNext Cell Lysis Buffer (10×) 12 20.8 0.5×
Murine RNase Inhibitor 6 10.4 -
Proteinase K (80 units/mL) 24 41.5 8 units/mL
NEBNext Single Cell RT Primer Mix 24 41.5 -
Water 174 301 -
Total volume 240 415.2