Component	Volume per reaction (μL)	Volume for 96 reactions with overage (μL)	Final concentration in PCR reaction
Tris-HCl, pH 8.4 (1 M)	0.18	21.6	15 mM
KCl (1 M)	0.3	36	25 mM
MgCl2 (1 M)	0.048	5.76	4 mM
dNTPs (25 mM each)	0.192	23.04	0.4 mM each
Rd1.AV.x1 / Rd1.BV.x1 primer mix (5 μM each)	0.12	14.4	50 nM each
RNase H2 (20 mU/μL)	0.3	36	0.5 mU/μL
Hot Start Taq DNA Polymerase (5 units/μL)	0.48	57.6	0.2 units/μL
Water	0.38	45.6	-
Total volume	2	240