| Component | Volume for 96-well plate with overage (μL) | Volume for 384-well plate with overage (μL) | Final concentration in RT reaction |
|---|---|---|---|
| NEBNext Single Cell RT Buffer (4×) | 120 | 207.5 | 1× |
| AAPV Proteinase K inhibitor (25 mM) | 9.6 | 16.6 | 0.5 mM |
| NEBNext Template Switching Oligo | 24 | 41.5 | - |
| NEBNext Single Cell RT Enzyme Mix | 48 | 83 | - |
| Water | 38.4 | 66.4 | - |
| Total volume | 240 | 415 | |
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