Table 5 |.

Enumeration of TCR-alpha and -beta TCR clonotypes detected in 384 putative tumor-specific T cells.

	1 Alpha + 1 Beta	2 Alpha + 1 Beta	1 Alpha + 2 Beta	1 or 2 Alpha Only	1 or 2 Beta Only	Multiple Alpha and Beta	No Alpha and Beta
Number of cells	289	49	6	5	17	1	17
Total cells	344			40

In each single cell, and separately for alpha and beta, detected clonotypes are ranked by number of CDR3 reads. Nonproductive CDR3 sequences with stop codon or frame shift are ignored. If less than 100 CDR3 reads are counted for the predominant sequence, then no alpha/beta clonotype is reported for that cell. If the CDR3 reads for the predominant sequence are 20× or greater than the second sequence, one alpha/beta clonotype is reported for that cell. If the sum of the CDR3 reads for the first and second sequences is 20× or greater than the third sequence, two alpha/beta clonotypes are reported for that cell. Any remaining cells are reported as multiple. If a cell has two alpha clonotypes and two beta clonotypes, it is also reported as multiple.