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. Author manuscript; available in PMC: 2020 Apr 29.
Published in final edited form as: Nat Protoc. 2019 Jul 24;14(8):2571–2594. doi: 10.1038/s41596-019-0195-x
 Component Volume for 96-well plate with overage (μL) Volume for 384-well plate with overage (μL) Final concentration in PCR reaction
NEBNext Single Cell cDNA PCR Master Mix (2×) 1200 2065 1×
NEBNext Single Cell cDNA PCR Primer 48 82.6 -
Water 672 1156.4 -
Total volume 1920 3304