| Component | Volume for 96-well plate with overage (μL) | Volume for 384-well plate with overage (μL) | Final concentration in PCR reaction |
|---|---|---|---|
| Tris-HCl, pH 8.4 (1 M) | 10.8 | 36.9 | 15 mM |
| KCl (1 M) | 18 | 61.5 | 25 mM |
| MgCl2 (1 M) | 2.9 | 9.84 | 4 mM |
| dNTPs (25 mM each) | 11.5 | 39.36 | 0.4 mM each |
| Rd1.AV.x1 / Rd1.BV.x1 primer mix (5 μM each) | 7.2 | 24.6 | 50 nM each |
| Rd2.AC.x1 / Rd2.BC.x1 primer mix (5 μM each) | 7.2 | 24.6 | 50 nM each |
| RNase H2 (20 mU/μL) | 18 | 61.5 | 0.5 mU/μL |
| Hot Start Taq DNA Polymerase (5 units/μL) | 28.8 | 98.4 | 0.2 units/μL |
| Water | 135.6 | 463.3 | - |
| Total volume | 240 | 820 | |
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