| Component | Volume per tube (μL) | Volume for 12 tubes with overage (μL) | Final concentration in RT reaction |
|---|---|---|---|
| NP-40 (10%) | 1 | 15 | 0.25% |
| dNTPs (25 mM each) | 0.8 | 12 | 0.5 mM |
| Rd2.UM7.AC primer (10 μM) | 0.84 | 12.6 | 210 nM |
| Rd2.UM7.BC primer (10 μM) | 0.28 | 4.2 | 70 nM |
| Water | 7.08 | 106.2 | - |
| Total volume | 10 | 150 | |
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