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. Author manuscript; available in PMC: 2020 Apr 29.
Published in final edited form as: Nat Protoc. 2019 Jul 24;14(8):2571–2594. doi: 10.1038/s41596-019-0195-x
 Component Volume per reaction (μL) Volume for 96 reactions with overage (μL) Final concentration in RT reaction
RT Buffer (5×) 0.8 96 1×
Dithiothreitol (1 M) 0.04 4.8 10 mM
RNaseOUT (40 units/μL) 0.08 9.6 0.8 units/μL
Superscript II Reverse Transcriptase (200 units/μL) 0.02 2.4 1 units/μL
Water 1.06 127.2 -
Total volume 2 240