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. 2020 Mar 10;122(9):1333–1341. doi: 10.1038/s41416-020-0781-2

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© The Author(s), under exclusive licence to Cancer Research UK 2020

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Fig. 3 — a Flow cytometry of GPC-1 expression in a GPC-1-knockdown cell line (BxPC-3 KD-2-23) and a control cell line (BxPC-3 NC-11) using anti-GPC-1 monoclonal antibody. b Quantitative reverse transcription-PCR analysis of GPC-1 mRNA levels relative to β-actin in a GPC-1-knockdown cell line (BxPC-3 KD-2-23) and control cell lines (BxPC-3 and BxPC-3 NC-11). c BxPC-3, BxPC-3 NC-11 and BxPC-3 KD-2-23 were treated with GPC-1-ADC for 144 h. GPC-1-ADC had a lower growth inhibition effect of the GPC-1-knockdown cell line (BxPC-3 KD-2-23) than the parent BxPC-3 and the negative control cell line (BxPC-3 NC-11).