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. 2020 Apr 28;10:7139. doi: 10.1038/s41598-020-64004-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction of apoptosis after exposure to increasing concentrations of α-LA (A) FaO cells were exposed to 100, 250 and 500 µM α-LA for 24, 48 and 72 hours and subjected to Hoechst 33258 nuclear staining; with a are indicated stained attached cells and with b apoptotic detached cells. (B) Percentage of apoptotic cells (Apoptotic Index). Data are shown as means ± S.E. Assay was performed in quadruplicate. Significantly different from control cells for p < 0.05*; p < 0.01 **; p < 0.001***.